糞便検体のプライマーについて
糞便の次世代シーケンス・アンプリコン解析において、当社開発の細菌・アーキア一括プライマーセットがGoldstandard method(s)として論文で紹介されました1,2)。
細菌・アーキア一括プライマーセットは、多くの論文で採用されています3-7)。そのため当社では、生体由来検体 (A 区分) の検体に関しましては、細菌・アーキア一括プライマーセットを推奨します。
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- 2) Ferrer, M., Raczkowska, B. A., Martinez‐Martinez, M., Barbas, C. & Rojo, D. (2017). Phenotyping of gut microbiota: Focus on capillary electrophoresis. Electrophoresis DOI: 10.1002/elps.201700056
- 3) Hari, A. R., Katuri, K. P., Logan, B. E. & Saikaly, P. E. (2016). Set anode potentials affect the electron fluxes and microbial community structure in propionate-fed microbial electrolysis cells. Sci Rep 6, 38690
- 4) Barthels, C., Ogrinc, A., Steyer, V., Meier, S., Simon, F., Wimmer, M., Blutke, A., Straub, T., Zimber-Strobl, U. & Lutgens, E. (2017). CD40-signalling abrogates induction of RORγt+ Treg cells by intestinal CD103+ DCs and causes fatal colitis. Nat Commun 8, 14715.
- 5) McAnulty, M. J., Poosarla, V. G., Kim, K.-Y., Jasso-Chavez, R., Logan, B. E. & Wood, T. K. (2017). Electricity from methane by reversing methanogenesis. Nat Commun 8, 15419.
- 6) Cahill, A. G., Steelman, C. M., Forde, O., Kuloyo, O., Ruff, S. E., Mayer, B., Mayer, K. U., Strous, M., Ryan, M. C. & Cherry, J. A. (2017). Mobility and persistence of methane in groundwater in a controlled-release field experiment. Nat Geosci 10, 289–294.
- 7) Li, M., Zhou, H., Pan, X., Xu, T., Zhang, Z., Zi, X. & Jiang, Y. (2017). Cassava foliage affects the microbial diversity of Chinese indigenous geese caecum using 16S rRNA sequencing. Sci Rep 7, 45697.
